Trihybrid Cross Calculator - Punnett Square (2024)

The trihybrid cross calculator creates a Punnett square with 3 traits and 6 alleles.
Our tool will also supply you with a detailed probability of all possible genes & traits combinations, as well as the short explanation of the most essential rules that we need to follow in genetics. 🧬

On top of that, we'll show you:

  • How to do a trihybrid cross.
  • How to create trihybrid cross ratios for both genotype and phenotype of the expected offspring.

💡 Dig into the topic:

Classic Punnett square calculator (1 trait) 💠
Dihybrid cross Punnett square calculator (2 traits)

What is a trihybrid Punnett square?

Let's start with the basics — the Punnett square is a simple, 4-boxes board that allows us to show the inheritance of a given trait.

♂️\♀️

A

a

A

AA

Aa

a

Aa

aa

Unfortunately, we, humans, just love to make things complicated. We decided to calculate & show the inheritance of 3 or more traits — and that's when our trihybrid cross Punnett square calculator came in handy.

The three traits Punnett square is a minimalist's nightmare — it consists of 64 boxes and depends on 12 alleles of 3 genes. To compute the offspring's genotype, we use information about two parents, each having exactly 6 alleles.

💡 Let's imagine that a mother of a child has black, curly hair and brown eyes, while the father's hair is red, straight, and his eyes are brown.

When we use the trihybrid Punnett square generator, we can tell the exact probability that the child will inherit all 3 traits from its mother or father and determine the frequency of all their combinations.

Trihybrid Punnett square is not just a nice presentation of data — it allows us to compute both the trihybrid cross ratios and the percentage of a given trait version's probability (e.g., we answer the question: what's the probability that my child will inherit all 3 of these traits?).

Want to see a Punnett square in action? 🤓 Check the blood type calculator.

How to use the trihybrid cross calculator?

This kind of Punnett square works only for autosomal genes, inherited by Mendelian rules. Follow our short instructions to get the fullest out of our trihybrid cross-ratio calculator!

  1. Choose the mother's traits. You may take one of three options:

    • aa — Homozygous recessive; both of the mother's alleles are recessive.
    • AA — Homozygous dominant; both of the mother's alleles are dominant.
    • Aa — Heterozygous; one of the mother's alleles is dominant, and one is recessive.

    Repeat the process for traits B and C.

  2. Follow the same pattern for father's traits.

  3. Enjoy your results! 🎉 Our trihybrid cross-ratio calculator will display:

    a) Occurrence of a given genotype in percentages;

    b) A ready-to-go Punnett square with 3 traits; and

    c) A table of how genotype affects the phenotype.

How to do a trihybrid cross Punnett square?

The three trait Punnett square might be quite a challenge! Just to remind you, we're dealing with:

  • A giant, 8×8 table of results;
  • 64 possible sets of crosses in each try;
  • 27 possible genotypes;
  • 8 possible mother's alleles combinations;
  • 8 possible father's alleles combinations; and
  • 729 possible Punnett square trihybrid cross examples!

That's why our trihybrid cross calculator is an essential must. 😱 However, we still decided to place here some instructions to let you better understand the process.

  1. First, you need to choose all your three traits and their alleles — dominant (AA), recessive (aa), mixed (Aa). You need to repeat the process 6 times, for each of the mother's and father's trait.

  2. Second, you find the possible alleles combinations of a given parent.

    If your mother's alleles are: aaBbCC, their possible combinations are:

    • aBC
    • abC

    Repeat the process for the second parent.

  3. Third, combine your 1st parent's possible combinations with your 2nd parent's possible combinations, using a Punnett square with 3 traits and 64 fields.

Here's the Punnett square trihybrid cross example for your reference:

♂️\♀️

ABC

ABc

AbC

Abc

aBC

aBc

abC

abc

ABC

AABBCC

AABBCc

AABbCC

AABbCc

AaBBCC

AaBBCc

AaBbCC

AaBbCc

ABc

AABBCc

AABBcc

AABbCc

AABbcc

AaBBCc

AaBBcc

AaBbCc

AaBbcc

AbC

AABbCC

AABbCc

AAbbCC

AAbbCc

AaBbCC

AaBbCc

AabbCC

AabbCc

Abc

AABbCc

AABbcc

AAbbCc

AAbbcc

AaBbCc

AaBbcc

AabbCc

Aabbcc

aBC

AaBBCC

AaBBCc

AaBbCC

AaBbCc

aaBBCC

aaBBCc

aaBbCC

aaBbCc

aBc

AaBBCc

AaBBcc

AaBbCc

AaBbcc

aaBBCc

aaBBcc

aaBbCc

aaBbcc

abC

AaBbCC

AaBbCc

AabbCC

AabbCc

aaBbCC

aaBbCc

aabbCC

aabbCc

abc

AaBbCc

AaBbcc

AabbCc

Aabbcc

aaBbCc

aaBbcc

aabbCc

aabbcc

The parent's alleles look as follows:

  • Mother: AaBbCc
  • Father: AaBbCc

Try our trihybrid cross calculator and find out the other 728 options!

FAQ

What is a trihybrid cross Punnett square?

A trihybrid cross is a type of Punnett square generated for 3 traits. This kind of Punnett square is a table of 64 boxes, created with the combinations of 6 mother's and 6 father's alleles.

What is the trihybrid cross Punnett square used for?

Trihybrid cross Punnett square is used to establish the probability of the combination of 3 different traits. For example, we may find out the chances of giving birth to a child with blue eyes, dark hair, and B blood type.

How many boxes are there in the trihybrid cross Punnett square?

There are 64 boxes in a trihybrid cross Punnett square. A Punnett square with 3 traits also contains:

  • A giant, 8×8 table of results;
  • 27 possible genotypes;
  • 8 possible mother's alleles combination;
  • 8 possible father's alleles combinations; and
  • 729 possible trihybrid cross versions!

How to calculate genotype probability?

It's easier than it seems!

  1. Do the Punnett square.

  2. Count how many times a given genotype is present in your table (e.g., 2).

  3. Find out the total number of combinations in a table (e.g., 64).

  4. Divide the number of your genotype by the total number of combinations (e.g., 2/64). To make it a percentage, multiply it by 100.

    Genotype probability = Number of genotypes in the table/ total number of combinations × 100

  5. Yay, you got it! 🎉

Trihybrid Cross Calculator - Punnett Square (2024)

FAQs

How to calculate the probability of a trihybrid cross? ›

Consider the following example of a trihybrid:
  1. Consider the probability of Aa from an Aa x Aa cross is 1/2, Same for Bb and Cc.
  2. Multiply all the probabilities (for Aa x Bb x Cc): 1/2 x 1/2 x 1/2 = 1/8.

What is the ratio of the Punnett squares in a Trihybrid cross? ›

With three unlinked genes, each parent can produce 8 different types of gametes, which generates 64 possible genotypic combinations. The phenotypic ratio of the trihybrid cross is 27:9:9:9:3:3:3:1. These are the phenotypes or the visible characters of the offsprings at the end of trihybrid cross.

What is an example of a Trihybrid cross? ›

A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs.

How do you make a cross Punnett square? ›

To make a Punnett square, start by drawing a box that's divided into 4 equal squares. Then, label the rows with one parent's genotype and the column's with the other parent's genotype. Then, label each square, starting with the letter to the left of the square followed by the letter above the square.

What is the Trihybrid method? ›

In a trihybrid cross, an individual with the genotype AaBbCc is crossed with another individual with the genotype aaBbCc. The F1 generation can have eight different genotypes: AaBbCc, AaBbcc, AaBbcc, AabbCc, aaBbCc, aaBbcc, aaBbcc, and aabbCc.

How to find genotypic ratio of trihybrid cross? ›

For more complex cases like trihybrid cross, a large Punnett square is obtained, making it very complicated to calculate the genetic ratios. For such cases, the forked line method is used to find the genetic ratios. In this example below, the trihybrid cross genotypic ratio is 27:9:9:9:3:3:3:1.

How to calculate Punnett square? ›

Count the total number of boxes in your Punnett Square. This gives you the total number of predicted offspring. Divide the (number of occurrences of the phenotype) by (the total number of offspring). Multiply the number from step 4 by 100 to get your percent.

What is the ratio of F2 in a Trihybrid cross? ›

For a trihybrid cross, the F2 phenotypic ratio is 27:9:9:9:3:3:3:1.

How many characters are considered in a Trihybrid cross? ›

A trihybrid cross involve three pair of characters, which will give rise to the F1 hybrids, which are heterozygous for three genes. How many types of gametes will be produced in both male and female?

What is the three point cross test? ›

In genetics, a three-point cross is used to determine the loci of three genes in an organism's genome. An individual heterozygous for three mutations is crossed with a homozygous recessive individual, and the phenotypes of the progeny are scored.

How to make a Punnett square diagram? ›

How to construct Punnett squares
  1. Determine the parental genotypes close genotypeThe alleles that an organism has for a particular characteristic, usually written as letters.. ...
  2. Split the alleles for each parent and add them into your Punnett square around the edges.

Top Articles
Latest Posts
Recommended Articles
Article information

Author: Duane Harber

Last Updated:

Views: 5367

Rating: 4 / 5 (71 voted)

Reviews: 86% of readers found this page helpful

Author information

Name: Duane Harber

Birthday: 1999-10-17

Address: Apt. 404 9899 Magnolia Roads, Port Royceville, ID 78186

Phone: +186911129794335

Job: Human Hospitality Planner

Hobby: Listening to music, Orienteering, Knapping, Dance, Mountain biking, Fishing, Pottery

Introduction: My name is Duane Harber, I am a modern, clever, handsome, fair, agreeable, inexpensive, beautiful person who loves writing and wants to share my knowledge and understanding with you.